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基本信息

載體類型: 

酵母單雜交載體 

載體抗性: 

Kan 

篩選標(biāo)記: 

TRP1 

啟動子: 

ADH1 

載體大小: 

7.9kb 

5' 測序引物: 

T7:TAATACGACTCACTATAGGG 

3' 測序引物: 

根據(jù)序列設(shè)計引物 

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質(zhì)粒圖譜和多克隆位點信息

注意:

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如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。


載體描述

pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.