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基本信息
載體類型: | 酵母單雜交載體 |
載體抗性: | Kan |
篩選標(biāo)記: | TRP1 |
啟動子: | ADH1 |
載體大小: | 7.9kb |
5' 測序引物: | T7:TAATACGACTCACTATAGGG |
3' 測序引物: | 根據(jù)序列設(shè)計引物 |
備注: | 如有侵權(quán)請產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196 |
質(zhì)粒圖譜和多克隆位點信息
注意:
客戶在我公司選擇構(gòu)建載體服務(wù)項目時,請自行提供母源載體。
如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。
載體描述
pGBKT7-Lam is a negative control plasmid that encodes a fusionof the human lamin C protein (a.a. 66–230) and the GAL4 DNA-BD (a.a. 1–147). The lamin C cDNA insert (GenBank Accession #M13451) was derived from the plasmid referenced in Bartel et al. (1993a). Plasmid modification was performed atBD Biosciences Clontech. Yeast cotransformed with pGBKT7-Lam and pGADT7-RecT, provide a measure of the backgroundthat is due to false-positive two-hybrid interactions. pGBKT7-Lam has not been sequenced.