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基本信息
載體類型: | 植物RNAi載體,VIGS載體 |
載體抗性: | Kan |
載體標簽: | EGFP;His |
啟動子: | 35S |
篩選標記: | / |
表達水平: | 高拷貝 |
載體大小: | 10380bp |
克隆方法: | 多克隆位點,限制性內(nèi)切酶 |
5' 測序引物: | primer F:5'-GGACATTGTTACTCAAGGAA-3' |
3' 測序引物: | primer R:5'-AACCTAAAACTTCAGACACG-3' |
備注: | 如有侵權請產(chǎn)權持有人盡快聯(lián)系法務15927229196 |
質粒圖譜和多克隆位點信息
注意:
客戶在我公司選擇構建載體服務項目時,請自行提供母源載體。
如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預期,也不承擔由此產(chǎn)生的任何責任。
載體說明
pTRV2-EGFP: Tobacco rattle virus (TRV) based cDNA clone was placed between the duplicated CaMV 35S promoter and the nopaline synthase terminator in a T-DNA vector. EGFP was introduced in pTRV2 before MCS.
載體序列
ORIGIN
1 ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat
61 gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc
121 ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac
181 cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt
241 aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac
301 gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg
361 ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt
421 tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg
481 tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg
541 aatcacttgc gctaatcaac atgggagata tgtacgatga atcatttgac aagtcgggcg
601 gtcctgctga cttgatggac gattcttggg tggaatcagt ttcgtggaaa gatctgttga
661 agaagttaca cagcataaaa tttgcactac agtctggtag agatgagatc actgggttac
721 tagcggcact gaatagacag tgtccttatt caccatatga gcagtttcca gataagaagg
781 tgtatttcct tttagactca cgggctaaca gtgctcttgg tgtgattcag aacgcttcag
841 cgttcaagag acgagctgat gagaagaatg cagtggcggg tgttacaaat attcctgcga
901 atccaaacac aacggttacg acgaaccaag ggagtactac tactaccaag gcgaacactg
961 gctcgacttt ggaagaagac ttgtacactt attacaaatt cgatgatgcc tctacagctt
1021 tccacaaatc tctaacttcg ttagagaaca tggagttgaa gagttattac cgaaggaact
1081 ttgagaaagt attcgggatt aagtttggtg gagcagctgc tagttcatct gcaccgcctc
1141 cagcgagtgg aggtccgata cgtcctaatc ccatggtgag caagggcgag gagctgttca
1201 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
1261 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
1321 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
1381 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
1441 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
1501 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
1561 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
1621 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
1681 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
1741 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
1801 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
1861 tcactctcgg catggacgag ctgtacaagt aaggatttaa ggacgtgaac tctgttgaga
1921 tctctgtgaa attcagaggg tgggtgatac catattcact gatgccatta gcgacatcta
1981 aatagggcta attgtgacta atttgaggga atttccttta ccattgacgt cagtgtcgtt
2041 ggtagcattt gagtttcgca atgcacgaat tacttaggaa gtggcttgac gacactaatg
2101 tgttattgtt agataatggt ttggtggtca aggtacgtag tagagtccca catattcgca
2161 cgtatgaagt aattggaaag ttgtcagttt ttgataattc actgggagat gatacgctgt
2221 ttgagggaaa agtagagaac gtatttgttt ttatgttcag gcggttcttg tgtgtcaaca
2281 aagatggaca ttgttactca aggaagcacg atgagcttta ttattacgga cgagtggact
2341 tagattctgt gagtaaggtt accgaattct ctagaaggcc tccatgggga tccggtaccg
2401 agctcacgcg tctcgaggcc cgggcatgtc ccgaagacat taaactacgg ttctttaagt
2461 agatccgtgt ctgaagtttt aggttcaatt taaacctacg agattgacat tctcgactga
2521 tcttgattga tcggtaagtc ttttgtaatt taattttctt tttgatttta ttttaaattg
2581 ttatctgttt ctgtgtatag actgtttgag atcggcgttt ggccgactca ttgtcttacc
2641 ataggggaac ggactttgtt tgtgttgtta ttttatttgt attttattaa aattctcaac
2701 gatctgaaaa agcctcgcgg ctaagagatt gttggggggt gagtaagtac ttttaaagtg
2761 atgatggtta caaaggcaaa aggggtaaaa cccctcgcct acgtaagcgt tattacgccc
2821 gtctgtactt atatcagtac actgacgagt ccctaaagga cgaaacggga gaacgctagc
2881 caccaccacc accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc
2941 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
3001 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
3061 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
3121 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
3181 agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa
3241 aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc
3301 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
3361 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
3421 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
3481 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
3541 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
3601 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
3661 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
3721 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
3781 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
3841 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
3901 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
3961 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
4021 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
4081 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
4141 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
4201 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
4261 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
4321 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
4381 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
4441 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
4501 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
4561 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
4621 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
4681 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
4741 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
4801 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
4861 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
4921 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
4981 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
5041 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
5101 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
5161 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
5221 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc
5281 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc
5341 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga
5401 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa
5461 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca
5521 ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag
5581 gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
5641 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
5701 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
5761 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
5821 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
5881 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
5941 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
6001 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
6061 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
6121 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
6181 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
6241 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
6301 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
6361 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
6421 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
6481 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
6541 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
6601 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
6661 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct
6721 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
6781 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
6841 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
6901 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc
6961 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
7021 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
7081 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
7141 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
7201 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
7261 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
7321 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
7381 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
7441 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
7501 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
7561 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
7621 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
7681 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
7741 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
7801 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
7861 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
7921 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
7981 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
8041 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
8101 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
8161 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
8221 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc
8281 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
8341 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
8401 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
8461 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
8521 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
8581 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
8641 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
8701 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
8761 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
8821 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
8881 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
8941 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
9001 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
9061 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
9121 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
9181 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
9241 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
9301 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
9361 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
9421 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
9481 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
9541 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcctaggcca ccatgttggg
9601 cccggcgcgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac actctcgtct
9661 actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag acttttcaac
9721 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg
9781 aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat aaaggaaagg
9841 ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga
9901 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgatg
9961 gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
10021 gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
10081 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga agatggcttc
10141 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc taccgacagt
10201 ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc
10261 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
10321 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg
//